AIP Publishing LLC
AIP Publishing LLC
  • pubs.aip.org
  • AIP
  • AIP China
  • University Science Books
  • Resources
    • Researchers
    • Librarians
    • Publishing Partners
    • Topical Portfolios
    • Commercial Partners
  • Publications

    Find the Right Journal

    Explore the AIP Publishing collection by title, topic, impact, citations, and more.
    Browse Journals

    Latest Content

    Read about the newest discoveries and developments in the physical sciences.
    See What's New

    Publications

    • Journals
    • Books
    • Physics Today
    • AIP Conference Proceedings
    • Scilight
    • Find the Right Journal
    • Latest Content
  • About
    • About Us
    • News and Announcements
    • Careers
    • Events
    • Leadership
    • Contact
  • pubs.aip.org
  • AIP
  • AIP China
  • University Science Books

Seeking Medical Insights in the Physics of Mucus

  • May 7, 2024
  • APL Bioengineering
  • News
Share:

In vitro testing platform offers a slimy window into gut health and disease treatments.

From the Journal: APL Bioengineering

This unique setup created a layer of mucus exposed to the air for easy access during experiments. The researchers used a magnetic wire and powerful magnets to study the properties of the mucus without disturbing it. Credit: Cai and Braunreuther et al.
This unique setup created a layer of mucus exposed to the air for easy access during experiments. The researchers used a magnetic wire and powerful magnets to study the properties of the mucus without disturbing it. Credit: Cai and Braunreuther et al.

WASHINGTON, May 7, 2024 – As much as we might not want to think about it, mucus is everywhere in our bodies. It coats our airways and our digestive systems and serves as a first line of defense against pathogens, a habitat for our microbiomes, and a conveyor belt for our insides to keep everything moving smoothly.

The front-line role of mucus means it is often the site of the first symptoms of infection or disease. Understanding how mucus changes, and what it changes in response to, can help diagnose illnesses and develop treatments. Designing a study to measure the physical properties of mucus, however, is nothing to sneeze at.

In APL Bioengineering, by AIP Publishing, researchers from Stanford University developed a system to grow mucus-producing intestinal cells and study the characteristics of the mucus in different conditions. Their platform can explore the effects of pathogens and help develop medications to combat them.

Currently, researchers looking to study intestinal mucus commonly use lab animals for their experiments. These animals would be given a pathogen or drug, and researchers would surgically remove the intestinal mucus to study the effects. This process is expensive, complex, and most importantly, deeply unpleasant for the animals.

In addition, the researchers say, the act of removing the mucus is likely to change how it behaves, making any obtained results unreliable.

“When people collect the mucus from the animal, they’ll pipette it off or scrape it off,” said author Margaret Braunreuther. “But what we found is that when we pipette the mucus layer from our cell culture, we see a very different behavior after that action. We think that the act of pipetting or scraping this very soft polymeric solution is resulting in more liquidlike behavior.”

The alternative process developed by the Stanford team involves growing a layer of intestinal cells on a laboratory plate exposed to air. These cells produce a layer of mucus that the researchers can easily access for testing. Using a magnetic wire, they could measure the consistency of the mucus without affecting its properties.

“We put this wire on the mucus layer of the live cells, and then we applied a magnetic force to this probe and measured its displacement,” said Braunreuther. “From the relationship between the applied force and the magnetic wire displacement, we can then calculate the rheological properties of the material.”

Using this setup, the team simulated an infection by a parasitic worm to observe how the mucus responds. This study serves as both a practical demonstration of their platform and a glimpse into how these infections could be treated.

The group is now exploring even more ways to leverage their approach for better mucus health.

“We have a parallel effort to study airway mucus, looking at conditions such as cystic fibrosis and acute asthma,” said author Gerald Fuller. “We also started a collaboration with a group in Berlin to develop drugs to restore a healthy mucus response.”

###

For more information:
Wendy Beatty
media@aip.org
301-209-3090

Article Title

Air-liquid intestinal cell culture allows in situ rheological characterization of intestinal mucus

Authors

Pamela C. Cai, Margaret Braunreuther, Audrey Shih, Andrew J. Spakowitz, Gerald G. Fuller, and Sarah C. Heilshorn

Author Affiliations

Stanford University


APL Bioengineering

APL Bioengineering is devoted to research at the intersection of biology, physics, and engineering. The journal publishes high-impact manuscripts specific to the understanding and advancement of physics and engineering of biological systems. APL Bioengineering is the new home for the bioengineering and biomedical research communities.

https://aip.scitation.org/journal/apb

Share:
  • Study Sheds Light on Cancer Cell ‘Tug-of-War’
  • Harnessing Green Energy from Plants Depends on their Circadian Rhythms

Keep Up With AIP Publishing

Sign up for the AIP newsletter to receive the latest news and information from AIP Publishing.
Sign Up

AIP PUBLISHING

1305 Walt Whitman Road,
Suite 110
Melville, NY 11747
(516) 576-2200

Resources

  • Researchers
  • Librarians
  • Publishing Partners
  • Commercial Partners

About

  • About Us
  • Careers 
  • Leadership

Support

  • Contact Us
  • Terms Of Use
  • Privacy Policy

© 2025 AIP Publishing LLC
  • 𝕏